2017-8-11 16:0:32025-05-25 04:47:59
locus
BSU08000
BSU_08000
geneLength
957
960
ec
outlinks
bsu
BSU08000
BSU_08000
Gene
Phenotypes of a mutant
a ''[[gene|mgtE]] [[gene|yfjQ]]'' mutant does not grow on rich media, this can be suppressed by the addition of 25 mM Mg2+, moreover the mutant grows well on minimal medim in the presence of citrate [Pubmed|24415722]
a ''[[gene|mgtE]] [[gene|yfjQ]]'' mutant does not grow on rich media, this can be suppressed by the addition of 25 mM Mg2+, moreover the mutant grows well on minimal medium in the presence of citrate (due to the activity of [[protein|CitM]]) [Pubmed|24415722]
Biological materials
Mutant
MGNA-C274 (yfjQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2272 NBRP B. subtilis, Japan]
MGNA-C274 (yfjQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2272 NBRP B. subtilis, Japan]
BKE08000 (Δ[[gene|yfjQ]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAACAACCCTCCACCTG, downstream forward: _UP4_TGAGGACAGCGAGCAGCTGT
BKK08000 (Δ[[gene|yfjQ]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAACAACCCTCCACCTG, downstream forward: _UP4_TGAGGACAGCGAGCAGCTGT
GP2853 (''[[gene|yfjQ]]''::''tet''), available in [SW|Jörg Stülke]'s lab
References
The protein
Protein family
CorA metal ion transporter (MIT) (TC 1.A.35) family (together with [[protein|CorA]]) (according to UniProt)
The protein
Paralogous protein(s)
[[this]]
The protein
Structure
[PDB|4I0U] (from Thermotoga maritima, 37% identity) [pubmed|23425532]